![agilent chemstation noise signal to noise lod agilent chemstation noise signal to noise lod](https://s3.manualzz.com/store/data/028101833_1-bad0b0867608cc89b76b378ad6572078-360x466.png)
All compounds could be resolved with baseline separation at 330 nm with maximum absorption. The analysis was carried out at a flow rate of 1.0 ml/min with the detection wavelength set at 330 nm the total run time was 55 min and the analytical temperature was 25 ℃. The mobile phase was filtered under vacuum through a 0.45 µm membrane filter and degassed prior to use. Mobile phase A(hereinafter referred as A) and Mobile phase B (hereinafter referred as B) were used as the mobile phase under gradient conditions (0 min, 85% A 5 min, 85% A 25 min, 75% A 30 min, 30% A and 60 min, 30% A) to analyze the samples. The Phenomenex Luna C18 analytical column (5 µm, 4.6 × 250 mm, Phenomenex) was tested with a guard column that was filled with the same stationary phase. The HPLC equipment was an Agilent 1260 HPLC system (Agilent, Santa Clara, CA, USA) with an Agilent binary pump, an auto-sampler, a column oven, and an Agilent 1260 photodiode array detector. 14 15 But the physicochemical simultaneous analysis using the chemical profiling of A.
![agilent chemstation noise signal to noise lod agilent chemstation noise signal to noise lod](https://community.agilent.com/resized-image/__size/900x600/__key/communityserver-wikis-components-files/00-00-00-01-79/pastedimage1621586418009v1.png)
12 13 In addition, the studies on the identification of chemical constituents to compare A. Currently, the methods of identifying Peucedani Radix are reported as molecular genetic identification, external morphological classification through organoleptic test, and internal morphological identification using a microscope. decursiva to prevent their mixed use in the distribution process, and to clearly discriminate origin species for the quality control in the distribution of medicinal herbs as it is highly likely that herbal medicinal materials derived from similar plants belonging to the same genus or their adulterations are distributed together. 11 Therefore, it is needed to contribute to the correct determination of P. Keywords: Peucedani Radix, Angelica decursiva, Peucedanum praeruptorum, coumarin, HPLC-DADĮspecially, as Peucedani Radix is dispersed in mountain slopes or grasslands mainly in the whole Asian regions, it has been actively distributed among countries. praeruptorum and contribute to quality control. Therefore, it is possible to distinguish between A. Also, the pattern recognition analysis based on HPLC indicates that all of the samples were largely clustered into two groups. praeruptorum were clearly classified by the quantification of four major coumarins in extracts. This method was fully validated for linearity, accuracy, precision, recovery, and limit of detection and quantification. Nodakenin ( 1), nodakenetin ( 2), praeruptorin A ( 3), and praeruptorin B ( 4) were separated with a Phenomenex Luna C18 column (5 µm, 4.6 × 250 mm) under the gradient conditions using distilled water with 0.1% phosphoric acid and acetonitrile with 0.1% phosphoric acid as the mobile phase, at a flow rate of 1.0 ml/min and a detection wavelength of 330 nm. For quantitative analysis, four major coumarins contained in these medicinal plants were assessed. The coumarins contained in Peucedani Radix were quantitatively analyzed using HPLC-DAD to develop a simultaneous determination for the quality control of A. Peucedani Radix is the root of Angelica decursiva Franchet et Savatier (= Peucedanum decursivum Maximowicz) or Peucedanum praeruptorum Dunn in several Asian countries.